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max in binding experiments, respectively. Statistical Examination of personal representative curves was executed by various t

Considerable genotype variations in the quantity of synapses are indicated in italics. Structured illumination microscopy of WT and KI midbrain slices was utilized to quantify the colocalization of the outlined proteins inside SERT-constructive synapses.

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Only homozygous animals had been used in the analyses, even though heterozygous mice ended up operate in behavioral experiments. 3 cohorts of eleven–sixteen mice underwent the first battery of behavioral assays for a total of 42 animals. All mice had been originally housed with their littermates of the same sexual intercourse from the Vanderbilt Murine Neurobehavioral Core, with controlled 12 h gentle/dark cycles, freely available food and h2o, and managed temperature and humidity. The experimenter was originally blinded to the genotype of every animal, and each animal underwent the identical battery of behavioral assessments to establish phenotype.

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